Synthesis and biological evaluation of a novel cardiolipin affinity matrix

MK Johns; MX Yin; SJ Conway; DEJE Robinson; LSM Wong; R Bamert; REH Wettenhall; AB Holmes

Journal article

Synthesis and biological evaluation of a novel cardiolipin affinity matrix

MK Johns, MX Yin, SJ Conway, DEJE Robinson, LSM Wong, R Bamert, REH Wettenhall, AB Holmes

Organic and Biomolecular Chemistry | ROYAL SOC CHEMISTRY | Published : 2009

DOI: 10.1039/b909306k

Abstract

Cardiolipin (1) is a dimeric phospholipid found in the mitochondrial membranes of both plants and animals. In order to understand better its role, we report the preparation of an immobilised analogue (2) using phosphoramidite chemistry; the probe has been used successfully to bind a recombinant protein containing a cardiolipin-binding domain. © 2009 The Royal Society of Chemistry.

University of Melbourne Researchers

Andrew Holmes Author

Related Projects (1)

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

We thank the BBSRC and EPSRC for financial support and for provision of the Swansea Mass Spectrometry Service. SJC thanks Hughes Hall (Cambridge) for a Research Fellowship. This work was supported in part by the Australian Research Council, Discovery Project, Grant DP0770668. General support was provided by CSIRO and VESKI (Victorian Endowment for Science, Knowledge and Innovation). We also thank Daiso Co., Ltd, Japan for the generous gift of (+)-1,2-O-isopropylideneglycerol.